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Original Article
Differences in Mobilization Efficiency between Small Children and Adults with Healthy Marrows
Clin Pediatr Hematol Oncol 2017;24:130-5.
Published online October 31, 2017
© 2017 Korean Society of Pediatric Hematology-Oncology and Korean Society for Pediatric Neuro-Oncology

Eun-kyung Shin, M.D.1, Do-Yeon Kim, M.D.1, Wee-Jin Rah, M.D.1, Hani Koh, M.D.1,2, Jin Young Suh, B.S.2, Misoo Chang, M.S.3, Eunwoo Nam, Ph.D.3 and Young-Ho Lee, M.D., Ph.D.1,2

1Department of Pediatrics, 2Blood and Marrow Transplantation Center, 3Biostatistical Consulting and Research Lab, Hanyang University College of Medicine, Hanyang University Medical Center, Seoul, Korea
Correspondence to: Young-Ho Lee
Department of Pediatrics, Hanyang University Hospital, 222-1 Wangsimni-ro, Seongdong-gu, Seoul 04763, Korea
Tel: +82-2-2290-8383 Fax: +82-2-2297-2380 E-mail: cord@hanyang.ac.kr 
Received September 25, 2017; Revised October 7, 2017; Accepted October 17, 2017.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: We compared the yields of mobilized PBSCs from single day of normal volume leukapheresis (NVL) in children and adults, and factors affecting the yields, to understand differences in mobilization efficiency between adults and small children with healthy marrows.Methods: This study involved 18 adult volunteer donors and 47 small children weighing less than 20 kg who participated in a clinical trial of cell therapy in children with cerebral palsy. Donor factors analyzed to identify predictors of the yield of apheresis included age, gender, weight and complete blood cell count (CBC) with differential counts as well as equipment parameters. Results: The yields of total nucleated cells (TNCs) and CD34cells in the apheresis products of the children were significantly lower than in those from healthy adults. However, the efficiency of recovery of PBSCs (total CD34 cell counts/TNCs) was significantly higher in small children (0.48±0.30%) than in adults (0.10±0.05%) (P<0.05). Multivariable analysis of adult donor factors showed that the processed volume and flow rate of apheresis were significantly associated with the yield of TNCs (P<0.05, for both), but not of CD34cells. However, in multivariable analysis of child donor factors, body weight and circulating WBC count on the day of apheresis were significantly associated with the yield of TNCs (P<0.05, for both) and of CD34cells (P<0.05, for both).Conclusion: The predictors of PBSC yields from a single day of NVL in adults and small children are different. Also mobilization is more effective in small children than in adults.
Keywords: Peripheral blood stem cell, Hematopoietic stem cell mobilization, Efficacy, Predictor, Children, Adults
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April 2018, 25 (1)
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