search for




 

Original Article
Effects of Low- or Moderate-dose Whole Body-X-ray Radiation on the Immune System of C57BL/6 Mice
Clin Pediatr Hematol Oncol 2018;25:50-5.
Published online April 30, 2018
© 2018 Korean Society of Pediatric Hematology-Oncology and Korean Society for Pediatric Neuro-Oncology

Yunmi Ko, M.S.1,2, Yeon Ho Jeong, Ph.D.2, Jun Ah Lee, M.D.3

1Division of Radiological Science and Clinical Research, Korea Cancer Center Hospital, Seoul, 2Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University, Chuncheon, 3Department of Pediatrics, Korea Cancer Center Hospital, Seoul, Korea
Correspondence to: Jun Ah LeeDepartment of Pediatrics, Korea Cancer Center Hospital, 75 Nowon-ro, Nowon-gu, Seoul 01812, Korea Tel: +82-2-970-1248 Fax: +82-2-970-1970 E-mail: junahlee@kcch.re.kr
Received March 29, 2018; Revised April 17, 2018; Accepted April 18, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Increase in the use of diagnostic imaging or occupational exposure to radiation have brought upon concerns on the safety and biological effects of low- or moderate-dose radiation. However, limited information is available on the effects of low or moderate dose radiation on human health.
Methods: Using C57BL/6 mice, we aimed to evaluate the biological effects of low- and moderate-dose radiation on the immune system. X-rays was chosen as a radiation source and we analyzed complete blood counts, various lymphocyte subsets and various cytokine levels after single fraction x-ray exposure (0.1 Gy, 1 Gy).
Results: No significant changes in the immunologic parameter of C57BL/6 mice were observed after radiation, except LIX (a cytokine equivalent to human CXCL5), that showed higher level after 0.1 Gy radiation compared to the control.
Conclusion: We observed that a single fraction of low or moderate dose of X-ray radiation does not cause significant changes in the immune system of C57BL/6 mice. Further studies are necessary to elucidate the mechanism underlying our results.
Keywords: Low-dose, X-ray, Radiation, Immune system, Mice
References
  1. Albert JM. Radiation risk from CT: implications for cancer screening. AJR Am J Roentgenol 2013;201:W81-7.
    Pubmed CrossRef
  2. Katsura M, Cyou-Nakamine H, Zen Q, et al. Effects of chronic low-dose radiation on human neural progenitor cells. Sci Rep 2016;6:20027.
    Pubmed KoreaMed CrossRef
  3. Song KH, Kim MH, Kang SM, et al. Analysis of immune cell populations and cytokine profiles in murine splenocytes exposed to whole-body low-dose irradiation. Int J Radiat Biol 2015;91:795-803.
    Pubmed CrossRef
  4. Nowosielska EM, Cheda A, Wrembel-Wargocka J, Janiak MK. Anti-neoplastic and immunostimulatory effects of low-dose X-ray fractions in mice. Int J Radiat Biol 2011;87:202-12.
    Pubmed CrossRef
  5. Lall R, Ganapathy S, Yang M, et al. Low-dose radiation exposure induces a HIF-1-mediated adaptive and protective metabolic response. Cell Death Differ 2014;21:836-44.
    Pubmed KoreaMed CrossRef
  6. Frey B, Hehlgans S, Rodel F, Gaipl US. Modulation of inflammation by low and high doses of ionizing radiation: Implications for benign and malign diseases. Cancer Lett 2015;368:230-7.
    Pubmed CrossRef
  7. Park B, Yee C, Lee KM. The effect of radiation on the immune response to cancers. Int J Mol Sci 2014;15:927-43.
    Pubmed KoreaMed CrossRef
  8. Shimura N, Kojima S. Effects of low-dose-gamma rays on the immune system of different animal models of disease. Dose Response 2014;12:429-65.
    Pubmed KoreaMed CrossRef
  9. El-Saghire H, Michaux A, Thierens H, Baatout S. Low doses of ionizing radiation induce immune-stimulatory responses in isolated human primary monocytes. Int J Mol Med 2013;32:1407-14.
    Pubmed CrossRef
  10. Bogdándi EN, Balogh A, Felgyinszki N, et al. Effects of low-dose radiation on the immune system of mice after total-body irradiation. Radiat Res 2010;174:480-9.
    CrossRef
  11. Goudarzi M, Mak TD, Chen C, Smilenov LB, Brenner DJ, Fornace AJ. The effect of low dose rate on metabolomic response to radiation in mice. Radiat Environ Biophys 2014;53:645-57.
    Pubmed KoreaMed CrossRef
  12. Tang FR, Loke WK, Khoo BC. Low-dose or low-dose-rate ionizing radiation-induced bioeffects in animal models. J Radiat Res 2017;58:165-82.
    CrossRef
  13. Shimura N, Kojima S. Effects of low-dose-gamma rays on the immune system of different animal models of disease. Dose Response 2014;12:429-65.
    Pubmed KoreaMed CrossRef
  14. Borzoueisileh S, Monfared AS, Abediankenari S, Mostafazadeh A, Khosravifarsani M. The effects of residence duration in high background radiation areas on immune surveillance. J Nat Sci Biol Med 2013;4:218-22.
    Pubmed KoreaMed CrossRef
  15. Hellweg CE. The nuclear factor kB pathway: a link to the immune system in the radiation response. Cancer Lett 2015;368:275-89.
    Pubmed CrossRef
  16. Ha CT, Li XH, Fu D, et al. Circulating interleukin-18 as a biomarker of total-body radiation exposure in mice, minipigs, and nonhuman primates (NHP). PLoS One 2014;9:e109249.
    Pubmed KoreaMed CrossRef
  17. Wang C, Oshima M, Sashida G, et al. Non-lethal ionizing radiation promotes aging-like phenotypic changes of human hematopoietic stem and progenitor cells in humanized mice. PLoS One 2015;10:e0132041.
    Pubmed KoreaMed CrossRef
  18. Rödel F, Fournier C, Wiedemann J, et al. Basics of radiation biology when treating hyperproliferative benign diseases. Front Immunol 2017;8:519.
    CrossRef
  19. Large M, Hehlgans S, Reichert S, et al. Study of the anti-inflammatory effects of low-dose radiation: the contribution of biphasic regulation of the antioxidative system in endothelial cells. Strahlenther Onkol 2015;191:742-9.
    Pubmed CrossRef
  20. Dawes JM, Calvo M, Perkins JR, et al. CXCL5 mediates UVB irradiation-induced pain. Sci Transl Med 2011;3:90ra60.
    Pubmed KoreaMed CrossRef
  21. Reichert O, Kolbe L, Terstegen L, et al. UV radiation induces CXCL5 expression in human skin. Exp Dermatol 2015;24: 309-12.
    Pubmed CrossRef
  22. Song J, Wu C, Zhang X, Sorokin LM. In vivo processing of CXCL5 (LIX) by matrix metalloproteinase (MMP)-2 and MMP-9 promotes early neutrophil recruitment in IL-1b-induced peritonitis. J Immunol 2013;190:401-10.
    Pubmed CrossRef
  23. Begley LA, Kasina S, Mehra R, et al. CXCL5 promotes prostate cancer progression. Neoplasia 2008;10:244-54.
    Pubmed KoreaMed CrossRef


October 2018, 25 (2)
Full Text PDF
Citation
Send to a friend
Twitter
Facebook

Cited By Articles
  • CrossRef (0)

Author ORCID Information
  • Jun Ah Lee 

Funding Information